Destination: Santa Fortuna

Using this Hitman season 2 walkthrough you can achieve the following challenges in Santa Fortuna for Three-Headed Serpent - Master Difficulty

Silent Assassin, Suit Only
Silent Assassin
Silent Assassin Suit Only
Mind The Step (Assassinate Jorge Franco by pushing him off a cliff as he searches for rare flowers)
A Bite Out Of Crime (Assassinate Rico Delgado by pushing him into the hippo pool)
Feedin' Time (Make the hipo feel better)
Salt the Earth (Destroy Franco's rare flower sample)
Tasteless Traceless (Assassinate a target with the lethal poison)
No Evidence
Tactical Strike (Bronze trophy)
Ghost of the jungle (Bronze trophy)